human heart cdna library (TaKaRa)
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human heart cdna library
Human Heart Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+heart+cdna+library/Human+Heart+QUICK-Clone+cDNA/pm41891541-43-24-28
Average 94 stars, based on 295 article reviews
Human Heart Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+heart+cdna+library/Human+Heart+QUICK-Clone+cDNA/pm41891541-43-24-28
Average 94 stars, based on 295 article reviews
human heart cdna library - by Bioz Stars,
2026-09
94/100 stars
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Construct:Article Title: Identification and validation of an interaction between the E3 Ubiquitin Ligase WWP1 and the transcriptional co-activator WBP2 in the human heart. Article Snippet: .. Since there was no autoactivation of the GAL4 reporter genes, the AH109 yeast containing the pGBK-WWP1 construct was used as bait to screen a Article Title: Open‐channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage‐gated K v 1.5 channel Article Snippet: .. The full‐length cDNA encoding human WT KCNE1 (hKCNE1; GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"M26685","term_id":"186569","term_text":"M26685"}} M26685 ) subcloned into pcDNA3.1 was constructed using PCR from the Article Title: Elevation of propofol sensitivity of cardiac I Ks channel by KCNE1 polymorphism D85N. Article Snippet: Correspondence Akiko Kojima, Department of Anesthesiology, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192, Japan.. Email: akiko77@belle.shiga-med.ac.jp Background and Purpose: The slowly activating delayed rectifier K channel (IKs), composed of pore-forming KCNQ1 α-subunits and ancillary KCNE1 β-subunits, regulates ventricular repolarization in human heart.. Propofol, at clinically used concentrations, modestly inhibits the intact (wild-type) IKs channels and is therefore unlikely to appreciably prolong QT interval in ECG during anaesthesia. Article Title: Identification and Validation of an Interaction between the E3 Ubiquitin Ligase WWP1 and the Transcriptional Co-Activator WBP2 in the Human Heart Article Snippet: .. Since there was no autoactivation of the GAL4 reporter genes, the AH109 yeast containing the pGBK-WWP1 construct was used as bait to screen a cDNA Library Assay:Article Title: Identification and validation of an interaction between the E3 Ubiquitin Ligase WWP1 and the transcriptional co-activator WBP2 in the human heart. Article Snippet: .. Since there was no autoactivation of the GAL4 reporter genes, the AH109 yeast containing the pGBK-WWP1 construct was used as bait to screen a Article Title: Glyceraldehyde-3-Phosphate Dehydrogenase Interacts with Proapoptotic Kinase Mst1 to Promote Cardiomyocyte Apoptosis Article Snippet: Cells were transiently transfected with FuGENE 6 (Roche Applied Science) using the manufacturer's protocol. .. To identify novel proteins that interact with Mst1, we screened a Article Title: Open‐channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage‐gated K v 1.5 channel Article Snippet: .. The full‐length cDNA encoding human WT KCNE1 (hKCNE1; GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"M26685","term_id":"186569","term_text":"M26685"}} M26685 ) subcloned into pcDNA3.1 was constructed using PCR from the Article Title: Elevation of propofol sensitivity of cardiac I Ks channel by KCNE1 polymorphism D85N. Article Snippet: Correspondence Akiko Kojima, Department of Anesthesiology, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192, Japan.. Email: akiko77@belle.shiga-med.ac.jp Background and Purpose: The slowly activating delayed rectifier K channel (IKs), composed of pore-forming KCNQ1 α-subunits and ancillary KCNE1 β-subunits, regulates ventricular repolarization in human heart.. Propofol, at clinically used concentrations, modestly inhibits the intact (wild-type) IKs channels and is therefore unlikely to appreciably prolong QT interval in ECG during anaesthesia. Article Title: Involvement of CaMKII in the modulation of I Ks under oxidative stress in guinea pig sinoatrial node cells. Article Snippet: Our previous study found that Ca2+/calmodulin-dependent protein kinase II (CaMKII) potentiates the slow delayed rectifier K current (IKs) in sinoatrial node (SAN) pacemaker cells.. Recently, oxidative activation of CaMKII has emerged as a major cause of SAN dysfunction; however, its correlation with IKs regulation remains unclear.. In this study, we investigated the effect of hydrogen peroxide (H2O2) on IKs in SAN cells isolated from guinea pig heart. Article Title: TNNI3K, a Cardiac-Specific Kinase, Promotes Physiological Cardiac Hypertrophy in Transgenic Mice Article Snippet: Proteins were detected by Western blotting and stained with NBT/BCIP (Promega, Madison, WI, USA) or ECL detection reagents (Amersham Pharmacia Biotech). .. A full-length human TNNI3K cDNA, fused to the GAL4 DNA binding domain, was used as bait in a yeast two-hybrid screen of approximately 3×10 6 clones of a Article Title: Identification and Validation of an Interaction between the E3 Ubiquitin Ligase WWP1 and the Transcriptional Co-Activator WBP2 in the Human Heart Article Snippet: .. Since there was no autoactivation of the GAL4 reporter genes, the AH109 yeast containing the pGBK-WWP1 construct was used as bait to screen a Dominant Negative Mutation:Article Title: Glyceraldehyde-3-Phosphate Dehydrogenase Interacts with Proapoptotic Kinase Mst1 to Promote Cardiomyocyte Apoptosis Article Snippet: Cells were transiently transfected with FuGENE 6 (Roche Applied Science) using the manufacturer's protocol. .. To identify novel proteins that interact with Mst1, we screened a Polymerase Chain Reaction:Article Title: Open‐channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage‐gated K v 1.5 channel Article Snippet: .. The full‐length cDNA encoding human WT KCNE1 (hKCNE1; GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"M26685","term_id":"186569","term_text":"M26685"}} M26685 ) subcloned into pcDNA3.1 was constructed using PCR from the Article Title: Elevation of propofol sensitivity of cardiac I Ks channel by KCNE1 polymorphism D85N. Article Snippet: Correspondence Akiko Kojima, Department of Anesthesiology, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192, Japan.. Email: akiko77@belle.shiga-med.ac.jp Background and Purpose: The slowly activating delayed rectifier K channel (IKs), composed of pore-forming KCNQ1 α-subunits and ancillary KCNE1 β-subunits, regulates ventricular repolarization in human heart.. Propofol, at clinically used concentrations, modestly inhibits the intact (wild-type) IKs channels and is therefore unlikely to appreciably prolong QT interval in ECG during anaesthesia. Article Title: Involvement of CaMKII in the modulation of I Ks under oxidative stress in guinea pig sinoatrial node cells. Article Snippet: Our previous study found that Ca2+/calmodulin-dependent protein kinase II (CaMKII) potentiates the slow delayed rectifier K current (IKs) in sinoatrial node (SAN) pacemaker cells.. Recently, oxidative activation of CaMKII has emerged as a major cause of SAN dysfunction; however, its correlation with IKs regulation remains unclear.. In this study, we investigated the effect of hydrogen peroxide (H2O2) on IKs in SAN cells isolated from guinea pig heart. Expressing:Article Title: Elevation of propofol sensitivity of cardiac I Ks channel by KCNE1 polymorphism D85N. Article Snippet: Correspondence Akiko Kojima, Department of Anesthesiology, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192, Japan.. Email: akiko77@belle.shiga-med.ac.jp Background and Purpose: The slowly activating delayed rectifier K channel (IKs), composed of pore-forming KCNQ1 α-subunits and ancillary KCNE1 β-subunits, regulates ventricular repolarization in human heart.. Propofol, at clinically used concentrations, modestly inhibits the intact (wild-type) IKs channels and is therefore unlikely to appreciably prolong QT interval in ECG during anaesthesia. Article Title: Involvement of CaMKII in the modulation of I Ks under oxidative stress in guinea pig sinoatrial node cells. Article Snippet: Our previous study found that Ca2+/calmodulin-dependent protein kinase II (CaMKII) potentiates the slow delayed rectifier K current (IKs) in sinoatrial node (SAN) pacemaker cells.. Recently, oxidative activation of CaMKII has emerged as a major cause of SAN dysfunction; however, its correlation with IKs regulation remains unclear.. In this study, we investigated the effect of hydrogen peroxide (H2O2) on IKs in SAN cells isolated from guinea pig heart. Plasmid Preparation:Article Title: Elevation of propofol sensitivity of cardiac I Ks channel by KCNE1 polymorphism D85N. Article Snippet: Correspondence Akiko Kojima, Department of Anesthesiology, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192, Japan.. Email: akiko77@belle.shiga-med.ac.jp Background and Purpose: The slowly activating delayed rectifier K channel (IKs), composed of pore-forming KCNQ1 α-subunits and ancillary KCNE1 β-subunits, regulates ventricular repolarization in human heart.. Propofol, at clinically used concentrations, modestly inhibits the intact (wild-type) IKs channels and is therefore unlikely to appreciably prolong QT interval in ECG during anaesthesia. Binding Assay:Article Title: TNNI3K, a Cardiac-Specific Kinase, Promotes Physiological Cardiac Hypertrophy in Transgenic Mice Article Snippet: Proteins were detected by Western blotting and stained with NBT/BCIP (Promega, Madison, WI, USA) or ECL detection reagents (Amersham Pharmacia Biotech). .. A full-length human TNNI3K cDNA, fused to the GAL4 DNA binding domain, was used as bait in a yeast two-hybrid screen of approximately 3×10 6 clones of a Two Hybrid Screening:Article Title: TNNI3K, a Cardiac-Specific Kinase, Promotes Physiological Cardiac Hypertrophy in Transgenic Mice Article Snippet: Proteins were detected by Western blotting and stained with NBT/BCIP (Promega, Madison, WI, USA) or ECL detection reagents (Amersham Pharmacia Biotech). .. A full-length human TNNI3K cDNA, fused to the GAL4 DNA binding domain, was used as bait in a yeast two-hybrid screen of approximately 3×10 6 clones of a Clone Assay:Article Title: TNNI3K, a Cardiac-Specific Kinase, Promotes Physiological Cardiac Hypertrophy in Transgenic Mice Article Snippet: Proteins were detected by Western blotting and stained with NBT/BCIP (Promega, Madison, WI, USA) or ECL detection reagents (Amersham Pharmacia Biotech). .. A full-length human TNNI3K cDNA, fused to the GAL4 DNA binding domain, was used as bait in a yeast two-hybrid screen of approximately 3×10 6 clones of a |